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board as above, over a bottle in which Anophelines, 

 caught in a village or elsewhere, have been placed. 

 Remove the cotton plug and shake the bottle gently 

 to drive the insects out. Replace jar upon the pre- 

 pared stopper. Place in a dark spot. Next morning 

 remove the stopper and remove any dead mosquitoes 

 and ova by taking out the piece of paper. 



On the second night after the mosquitoes have 

 been collected, the bottle is ready for feeding experi- 

 ments. On the third day, generally, the mosquitoes 

 have no longer any blood remaining in the mid-gut, 

 and are ready for dissection. 



The glands of any mosquitoes that may die before 

 this may of course be dissected, if desired, on the chance 

 of finding sporozoits. 



In the use of village-caught Anopkelines, it must 

 be borne in mind that any subject upon which they 

 are fed is liable to a fresh infection. In the case of 

 natives (who sleep without hesitation in any village), 

 the employment of village-caught mosquitoes cannot, 

 however, be very prejudicial. 



The advantages of the above way of keeping 

 mosquitoes are : 



1. The mosquitoes will keep alive longer than 

 in any other way known to us. 



2. The immense convenience in feeding. 



3. Any mosquitoes that may have died in the 

 night can be recovered, and are not dried up. 



4. It is an extremely convenient way of obtaining 

 and examining the ova. 



5. Mosquitoes which have become feeble are 

 given the best possible chance of living, and will be 

 found resting all day on the piece of paper. 



If boxes and net-covered frames be used, an 

 enormous mortality usually results. The dead bodies 



