94 



this around the neck of the bottle with twine. The 

 bottle is then ready for feeding. 



At, or shortly after dark, take as many bottles as 

 may be desired to the ward or dormitory. Slightly 

 damp the forearm (or the calf) of the patient, and, 

 turning the bottle right side up, let the patient's 

 arm rest upon the mouth of the bottle (Fig. 36). 



In from a few minutes to half-an-hour or more 

 the bottle will be noticed to have splashes of blood 

 upon the bottom and sides (in the case of Anophelines 

 only). If possible wait till all the Anophelines have fed. 



Remove the bottle, invert upon a table, untie the 

 twine, and remove the netting. Replace the bottle 

 upon the prepared stopper. 



Repeat the process every night, allowing the 

 mosquitoes to feed by preference on the same case 

 throughout. 



Add clean water and a fresh piece of paper each 

 time the bottle is used. 



To PREPARE FED MOSQUITOES FOR DISSECTION 



After having fed the mosquitoes in a bottle for 

 a certain number of days, allow them to remain 

 undisturbed (merely changing the water, etc.) for 

 several days. 



Ascertain each day whether the mosquitoes have 

 completely got rid of the blood in the mid-gut. When 

 they are quite free from any dark colouration of the 

 ventral aspect of the abdomen they are ready for 

 dissection. 



N.B. If chloroform, and especially if tobacco smoke is used to 

 kill the mosquito, it is essential to well wash the jars before again 

 keeping mosquitoes in them. 



