g8 



and possibly the extreme anterior portion of the 

 abdomen will appear transparent (oesophageal diver- 

 ticulum, containing gas) (Fig. 37). 



To DISSECT OUT THE Mio-GuT (STOMACH) 



1. Pull off, with forceps, the legs and wings. 



2. Place the mosquito in a drop of salt solution 

 on a slide, and place the slide on a light background. 



Turn the mosquito upon its back and with a 

 needle make a nick in the chitin on both sides as near 

 the ' tail ' end as possible. Now place one needle on 

 the thorax and the other on the extreme end and pull 

 gently. 



3. After separating the segments a very short 

 distance, remove the preparation to a dark background. 

 Again draw apart and note the white viscera stretching 

 between them. Make steady traction until a central 

 rather transparent body is alone left between the two 

 portions of abdomen. 



Cut across the anterior attachments of the 

 mid-gut. 



4. Draw the body of the mosquito away from 

 the separated segments ; the mid-gut and sundry 

 other viscera will be left attached to the latter floating 

 in the salt solution. 



