104 



remains in the mid-gut (for methods of keeping alive 

 see p. 90). 



Prepare the mid-gut as described above. A con- 

 siderable number may prove negative, but a variable 

 percentage will be positive. Examine with one- 

 twelfth inch. 



Carefully note the presence of small collections 

 of pigment of the nature of malarial figment. By 

 careful focussing, the younger forms may be seen as 

 clear oval or round bodies, 6-7/1, in which the distinct 

 clearly defined pigment occurs. The more advanced 

 forms can scarcely be missed. It is necessary to bear 

 in mind the normal structures and the fact that, until 

 the parasite reaches a considerable size and has a very 

 sharply defined cyst wall, pigment, of the character 

 belonging to the species of parasite concerned, is 

 present. 



Fig. 40. (Left to right} Pericardial Cell, Fat-Body Cell, Swollen 

 Epithelium Cell, Diverticulum shewing Sporozoit-like appearance 



1. Zygotes of malignant tertian shew, when 

 young, a clump of pigment resembling black pepper 

 grains (Fig. 41). 



2. Zygotes of simple tertian shew yellowish 

 or golden pigment in wisps (Fig. 41). 



3. Zygotes of quartan shew rather coarse pigment 

 in a clump (Fig. 41). 



The older zygotes (40-60/4) are indistinguishable 



