as regards the species of parasite concerned. They 

 may shew : 



1. A very clear and distinct oocyst wall (adven- 

 titious). 



2. The formation of sporoblasts. ^ 



3. In still more developed forms, the sporoblasts 

 are seen to be surrounded by a radiating arrangement 

 of young sporozoits or blasts (Figs. 14 and 39). 



4. Fully developed forms are large cysts packed 

 with many hundreds of fine sickle-shaped bodies, and 

 if they are ruptured, these latter escape into the 

 surrounding fluid, and are readily distinguished with 

 a sixth-inch lens as sporozoits (Fig. 45). 



Fig. 41. (Left to right} Zy gates of Malignant Tertian, Simple 

 Tertian, Quartan, and Proteosoma 



To MAKE PERMANENT PREPARATIONS OF 

 ZYGOTES 



Method i. In case of a specimen shewing zygotes, 

 place a large drop of ten per cent, formalin on one 

 side of the coverglass, and draw this through by filter 

 paper placed on the other side. Repeat several times. 

 Remove excess of the formalin with filter paper. Seal 

 edges of coverglass first with glycerine jelly and then 

 with cement (p. 408). 



The zygotes will retain their appearance as seen 

 in the fresh specimen. 



Method 2. Run formalin through as in Method I. 



