Ill 



80 F. Prepare specimens of the glands, as described 

 above. Press with the point a needle on the coverglass, 

 so that the gland is ruptured, and the secretion poured 

 out as droplets into the surrounding fluid. 



Examine with one-sixth inch. If sporozoits are 

 present they are generally very numerous, and large 

 numbers of fine, very distinct curved rods, will be 

 easily seen with this power, lying throughout the fluid 

 around the gland and packed in large numbers in the 

 substance of the gland. Finally, examine with one- 

 twelfth inch (Fig. 45). 



The sporozoits have a mean length of 14^, and 

 vary between io/i and 20/4, and are 1-2/4 in width. 



Examination of Motion of Sporozoits. Dissect out 

 the glands and, when isolated cleanly, transfer to a 

 drop of human serum, previously got ready by allowing 

 blood to clot in a small tube. Three kinds of motion 

 may be observed : 



1. Formation of curves. 



2. Formation of ring-formed contractions. 



3. Locomotion. Forward motion. 

 Penetration of Red Cell by Sporozoits. This has 



not been seen in case of sporozoits of the salivary glands, 

 but has been observed twice by Schaudinn in the case 

 of sporozoits from a ruptured cyst in the stomach. 

 Repeat the observation by mixing a little blood with 

 sporozoits under a coverglass. 



To Prepare Permanent Preparations of Sporozoits. 

 Pressing firmly upon the coverglass, draw it along the 

 slide, so that a film is made on coverglass and slide. 



Dry by rapidly waving the slide and the coverglass 

 in the air. Fix both in alcohol, and stain with Roman- 

 owsky. Wash, dry, and examine without coverglass 

 with an oil immersion. 



The sporozoits appear as fusiform bodies with a 



