US 



3. Sporozoa. (a) Sausage - shaped bodies in 

 masses, sometimes in close connexion with the salivary 

 glands (Fig. 48). 



(b) Octosporozoa, consisting of eight small 

 sausage-shaped bodies in small cysts, enormous numbers 

 of which replace the yelk of the ovum (Fig. 48). 



(c) Gregarines. Occasionally on the outside of 

 the stomach or encysted in the malpighian tubes. In 

 the larvae, the worm-like gregarine will be found 

 actively motile in the malpighian tubules. 



4. Flagellates. In the rectum and hind-gut, 

 frequently in enormous numbers, and shewing, when 

 stained, large numbers of developmental forms (p. 362). 



5. Developing Embryos of Filaria. These are 

 seen as sausage-shaped bodies with a terminal spike 

 in the dissection of the salivary glands. In sections 

 of mosquitoes they are seen in the muscles, especially 

 in those of the thorax, and the developed embryos 

 occur in the labium or about the base of the neck. 



6. Micro-organisms and Sporozoa (Nosema) ? 

 In the oesophageal diverticula. 



To CUT SECTIONS OF MOSQUITOES 



The following combined celloidin-paraffin method 

 gives admirable results where it is required to preserve 

 intact, especially the chitinous parts, and by this method 

 and hardly any other it is possible to procure sections 

 of a hard proboscis like that, for instance, of Stomoxys 

 calcitrans. 



A difficulty that occurs with chitinous organisms 

 like flies, is the penetration of the fixative. So difficult 

 is it to get perfect fixation that it is advisable where 

 details of structure are being studied to remove the 

 part required and to fix by itself. 



I. Select Anophelines free from blood and kill 



