227 



Nudeo- Albumens. Filter the urine carefully ; 

 boil to remove albumen ; then add gradually excess 

 of strong acetic acid. A turbidity indicates nucleo- 

 albumens. 



BLOOD (HAEMOGLOBIN, ETC.) 



1. Examine Spectroscopically (Fig. 72). If the 

 bands of methaemoglobin or oxyhaemoglobin are seen, 

 confirm by adding ammonium sulphide when the 

 bands of reduced haemoglobin are got. 



2. Heller's Test. Make the urine strongly 

 alkaline with caustic soda ; boil ; the precipitate in 

 the presence of haemoglobin is bright red ; confirm 

 by dissolving the filtered precipitate in acetic acid, 

 a red solution is formed (spectroscopically this gives 

 the characteristic bands of haemachromogen). 



3. Guiacum Test. Equal parts of. tincture of 

 guiacum and oil of turpentine (which has been exposed 

 to the air) are taken ; add slowly to the urine. A blue 

 ring is formed at the junction layer. (Unreliable). 



METHAEMOGLOBIN 



The urine in blackwater fever when examined 

 early, most frequently contains blood pigment in this 

 form, later oxyhaemoglobin. This, according to 

 Hoppe-Seyler, also holds good for every urine with 

 haemoglobin in solution. 



The Characters of Methaemoglobin are : 

 In acid solution the oxyhaemoglobin bands are 

 weak or invisible. There is a band between C and D, 

 nearer the former. The band of acid haematin is 

 similar in position. It is, however, close to C. 



