233 



drug has of inducing attacks of haemoglobinuria 

 (blackwater fever) in patients resident in regions where 

 malaria is especially virulent, and where generally the 

 parasite form is the malignant tertian associated with 

 an extremely high endemic index of native (children) 

 malaria. 



Two hundred c.c. of urine are acidified with 

 some drops of sulphuric acid. A spoonful of solid 

 picric acid is then added. The solution is allowed to 

 stand for an hour and then filtered. The solution 

 should be quite clear and should give with a saturated 

 solution of picric acid no turbidity. If there is 

 difficulty in getting a clear filtrate add a trace of egg 

 albumen and filter again. The half-dry residue is 

 then digested in an Erlenmeyer flask with fifty c.c. of 

 3*0 per cent, soda solution for half an hour on the 

 water bath. Now add sixty c.c. chloroform ; shake 

 for two hours in a shaking apparatus. The solution of 

 chloroform is now removed by means of a separating 

 funnel and collected in a weighed flask. The flask 

 should have a long neck to prevent spurting. Evapor- 

 ate in a water bath and dry at I2OC. The residue 

 is quinine. The experimental error is only one to 

 two per cent. 



DETERMINATION OF THE PERIODICITY OF 

 PARASITE DEVELOPMENT 



The inspection of a temperature chart is not in 

 itself sufficient to determine the cycle of development 

 of a parasite. Thus, as is well known, a quotidian 

 temperature chart may be produced by a double 

 tertian (simple) or by a triple quartan infection. If 

 then, in the case of the double tertian, we made micro- 

 scopical examinations at definite intervals for forty- 



