3 M- 



The Male. Treat as in the case of the ungorged 

 female. Note the distended vasa deferentia and the 

 narrow alimentary diverticula (Fig. 101). 



The Nymph. To prepare specimens of the freshly 

 gorged nymph cut off, by. pressing with a sharp scalpel, 

 a thin slice from the posterior end, and passing the 

 scalpel from before backwards, press out the whole of 

 the contents into a drop of normal saline. Search for 

 separate organs with a low power lens. To obtain 

 specimens of the tissues of nymphs which have advanced 

 towards metamorphosis, treat as in the case of the 

 ungorged female, or better, cut off the anterior one- 

 third of the body by means of a sharp scalpel and 

 turn out its contents. Observe that a rough model 

 of the future adult in embryonic tissue is present. 



For observing developmental stages of Piroplasma, 

 make smear preparations of this tissue and look under 

 a low power for parts where salivary ducts and young 

 acini are forming. 



EXAMINATION OF TICKS 



Place a tick alive upon a slide and cover with a 

 second slide. Pass a rubber band over each end (or 

 tie with thread) so as to compress the tick into an 

 extended position suitable for examination. (Rubber 

 bands are easily made from a piece of rubber tubing). 



In this way note the characteristics of (a) the unfed 

 larva ; (b) the gorged larva ; (c) the unfed nymph ; 

 (d) the gorged nymph ; (e) the unfed female adult ; 

 (/) the unfed male adult ; (g) the gorged female adult 



(Fig. 94)- 



To Mount Ticks 



Method L (a) Place the ticks mounted as above 



