(slides, rubber bands and all) into absolute alcohol for 

 about an hour. 



(b) Remove the rubber bands and place iir the 

 tick in (CuSO 4 ) absolute alcohol for about another 

 hour. 



Clear in oil of cloves and lower into a large drop 

 of thickish balsam so as to avoid air bubbles. 



Method II. Kill by placing in boiling water, 

 then proceed as in Methods I, III, etc. The tick is 

 suitably extended by this method. 



Method III. (a) Boil the compressed tick for 

 from ten to thirty minutes, or even much longer, in 

 ten per cent, caustic alkali, r "until it looks quite trans- 

 parent. 



In the case of soft bodied ticks, especially gorged 

 nymphs and larvae, it is necessary to regulate the 

 pressure, or they may become ruptured either before 

 or after placing them in the solution. For nymphs and 

 larvae coverglasses may be used in place of slides. 



(b) Remove the tick and wash thoroughly in 

 water till quite free from alkali. 



(c) Stain in a saturated solution of fuchsin (if 

 desired) r wash in alcohol. 



(d) Dehydrate, clear and mount in balsam. 

 Method IV. For ticks that are already dry, boil 



in ten per cent, potash or soda till clear. Wash in 

 water, dehydrate in alcohol, clear in oil of cloves and 

 mount in balsam. (Stain in fuchsin after the boiling, 

 if necessary). 



It is often advisable to open the tick or remove 

 some of the dorsal integument in order to see the 

 mouth parts clearly (Newstead). 



Method V. To Cut Sections of Ticks. Take ticks, 

 as young as possible, best of all those that have only just 

 moulted, then proceed as on page 116. 



