336 



the effect. In these rosettes there is no loss of motility, 

 and the clusters may separate again. In cultures, e.g., 

 of T. lewisi, the trypanosomes are agglomerated by their 

 anterior ends (Fig. 107). 



w j 



Fig. 107. (i) Agglomeration of trypanosomes ; (2) Culture forms 

 (Herpetomonas] in rosette 



CULTIVATION OF TRYPANOSOMES 



Novy-MacNeal-Mathis Medium. i. Collect the 

 blood of any suitable animal, e.g., cow, rabbit, etc. 

 (strict asepsis is not absolutely necessary). 



2. Defibrinate by shaking up with wire, or glass 

 beads, etc. 



3. Add one part of blood to two of agar in a 

 number of tubes. 



4. Sterilize once or twice at iooC., and make 

 slopes. (Fide also p. 353.) 



5. Inoculate* the condensation water with a little 

 blood of the trypanosome to be grown, e.g., T. lewisi, 

 and keep at room temperature. 



