353 



blood, in equal parts, or two parts of blood and one 

 of agar. 



The blood maybe got by bleeding from the carotid, 

 or by inserting a trocar and cannula directly into the 

 heart, through the thorax, in an anaesthetized rabbit. 

 Collect in a sterile jar, shaking well with pieces of glass 

 rod or wire, etc. Mixing the blood and agar in flasks 

 or as ordinary c slopes,' and after covering with rubber 

 caps, place in the incubator, at 37, over night, to make 

 sure of sterility. (Fide also p. 336). 



Inoculate the tubes with rats' blood containing 

 T. lewisi, taken from the heart by means of a sterile 

 pipette, and incubate at 25 C. As soon as a good 

 growth (rosettes) is obtained, sub-cultures should be 

 made. 



The cultural forms vary in length from 1-2/4 to 

 60/4. Most characteristic are the rosettes, consisting 

 of large masses of trypanosomes with their flagella 

 centrally placed. The position of the blepharoplast 

 is anterior instead of posterior, and the undulating 

 membrane but little developed. The cultures are 

 infective, and Novy and MacNeal state that, after 

 passing the culture fluid through a Berkefeld filter, the 

 filtrate is still infective. 



In Muridae, we have also T. longocaudense in the 

 Indian Mus niveiventer, and T. duttoni in the Senegal 

 mouse Mus musculus, and T. blanch ardi in Myoxut 

 nitela, garden dormouse. T. myoxi in M. avellanarius, 

 red dormouse. T. bandicotti (? = T. lewisi) in the 

 bandicoot Nesokia gigantea. 



TRYPANOSOMES IN BATS 



(i) T. nicolleorum in Fes-pertilio kuhli and Myotis 

 murinus in Africa. A small (24/4) active form and a 

 large (30^) sluggish form are described. 



