3 6i 



Detection of Trypanosomes. The most certain 

 method is by culture ; this may be positive when 

 repeated blood examination has been negative. Dani- 

 lewsky states that, while rare in the blood (as is generally 

 the case), trypanosomes may be abundant in the bone 

 marrow. 



TRYPANOSOMES OF INVERTEBRATES 



1. T. grayi.p. 342. 



2. T. tullochi. p. 343. 



3. T. piscicolae. Common in leeches, together 

 with trypanoplasmata. 



4. T. christopbersi. Found in one specimen of 

 Eu. sanguineus (the Indian dog-tick). It is larger than 

 Herpetomonas and Crithidia forms. 25 by 2*4/4. 

 Flagellum 8-I2/*. U.M. well developed. Blepharo- 

 plast close to the nucleus. 



FLAGELLATES IN THE GUT OF INSECTS, ETC. 



Besides the true trypanosomes natural to the 

 tsetse fly already described, allied flagellates are found 

 in the gut of various insects. These are generally 

 assigned to two different genera, though the definition 

 of these genera is at present somewhat vague. 



Crithidia. Includes two forms : (a) short, oval 

 or pyriform flagellates, rounded posteriorly, somewhat 

 truncated anteriorly, with a short straight flagellum 

 ('pears'). The nucleus and blepharoplast are posterior. 



(b) Longer forms, rounded at each end or tapering 

 slightly at one end. (not at both as in Herpetomonas), 

 with a long flagellum ('cigars'). The nucleus is median 

 and the blepharoplast half-way between it and the 

 anterior end. The flagellum [may have a fine 

 extension of the periplast over it. There is no 



