3- Further observe that polynuclear leucocytes 

 contain only one or two of these bodies (Fig. 16) ; 

 large mononuclear leucocytes one to six (Fig. 17) ; 

 cells of an endothelial type one to twelve (Fig. 18) ; 

 large cells with a hyaline or finely granular or vacuo- 

 lated cytoplasm (macrophages) up to several hundreds 

 (Fig. 19). 



4. The parasites are approximately circular or 

 oval, 2*5-3*5 M m s i 7 A clearly outlined, and appear 

 to possess a distinct cuticle, as they retain their shape 

 and are rarely seen distorted in films. 



5. The two chromatin masses are characteristic, 

 the large one staining lightly and the small one intensely 

 with Romanowsky. The masses are usually situate 

 opposite each other in the short axis of the parasite. 

 The larger chromatin mass always forms part of the 

 periphery of the parasite. 



6. Most of the parasites contain one or two 

 vacuoles which may displace the cytoplasm of the 

 parasites to the periphery. 



7. Developmental forms. Division commences 

 at the thick end of the parasite, and the large chromatin 

 masses may be widely separated before the small 

 chromatin mass has begun to divide. As many as three 

 to six bodies are formed in this way, the large nuclei 

 being arranged peripherally, and the smaller centrally 

 (Figs. 7-11). 



OCCURRENCE IN PERIPHERAL BLOOD 



Parasites can be found in the blood in about 

 eighty per cent., if not in all cases. Properly spread 

 films (p. 19) must be used, especially as leucopenia is 

 so common, and the leucocytes carefully examined ; 

 for the parasites do not occur free. It may be necessary 

 to examine more than one film (Patton). 



AA 



