373 



Lymphatic glands. In those draining the area of a skin lesion 

 parasites are found. They occur in large cells in the lymph sinuses 

 and in cells of the reticulum. 



Development. Pattoirhas traced the development 

 of the parasite in the Indian bed bug Gimex macro- 

 cephalus up to the Herpetomonas stage, as seen in 

 cultures. 



Culture. Add blood taken by a splenic puncture 

 to a drop or two of ten per cent, sodium citrate in 

 a small test tube. Slight acidification with citric acid 



Fig. 113. (i) Parasite as seen in splenic blood; (2) Third 

 day, parasite enlarged ; (3) Fourth day, full size ; 



(4) Chromatin masses at the extremities ; 

 (5) Stumpy flagellate forms ; (6) Elongate flagellate forms 



assists development (Rogers). Incubate at 22 to 24C. 

 About the third day the parasites are as big as a red cell 

 and the nuclei are no longer peripheral (Fig. 113). 

 These forms may divide, forming groups, or flagella 

 may develop directly from the enlarged parasite. 

 About the fourth to sixth day elongate flagellates or 

 forms still stumpy appear. They resemble Herpeto- 

 monas forms. Leishman describes also i spirochaete ' 

 forms. 



