408 



2. Haematein. 



Haematein (puriss. ) 0*4 gr. 



Alum 5*g r - 



Glycerin 30*0 c.c. 



Water 70*0 c.c. 



Rub up the haematein with a little glycerin, and 

 add the other ingredients. The stain is ready for use 

 at once, and keeps well. 



3. Methylene Blue. For staining blood or 

 tissues, use a quarter per cent, watery solution of pure 

 methylene blue. 



4. Eosin. - - This is really Brom-eosin, i.e., 

 yellowish or water-soluble eosin. For blood work and 

 also for tissues is best used in the form of a half per cent, 

 solution in seventy per cent, alcohol. 



Eosin* 0-5 grm. 



Absolute alcohol - 70-0 c.c. 



Water 30*0 c.c. 



5. Ehrlictfs Triacid. - - Take H 2 O, 45 c.c. ; 

 glycerine, 10 c.c. ; alcohol, ninety per cent., 25 c.c. ; 

 and add in the following order : acid fuchsin, 3 grm. ; 

 orange G, 2 grm. ; methyl green, I grm. Stains 

 typically and keeps well. 



Iron Reaction (Haemosideriri) in Malarial Tissues. 



(1) Treat the sections after removing the paraffin 

 with two per cent, watery solution of potassium ferro- 

 cyanide for from five to twenty minutes. 



(2) Acid alcohol (HC1 I part, seventy per cent, 

 alcohol 100 parts) five to ten minutes. 



(3) Wash in water. 



(4) Counterstain with alum carmine. 



To Mount-Specimens in a Fluid Medium. Remove 

 all fluid from the edge of the coverglass. Seal with 

 plenty of melted glycerine jelly. Examine next day to 



* Use ' pure French ' eosin or a spirit-soluble eosin. 



