vi CONTENTS 



CHAPTER V. 



Preparation of Media Bouillon, Gelatin, Agar-agar, Potato, 

 Blood-serum, Blood-serum from Small Animals, Milk, 

 Litmus-whey Milk, Durham's Peptone Solution, Lactose 

 Litmus-agar, Loffler's Blood-serum Mixture, the Serum- 

 water Media of Hiss, Guarniari's Gelatin-agar Mixture . . 108 



CHAPTER VI. 



Preparation of the Tubes, Flasks, etc., in which the Media are to 



be Preserved ................ .133 



CHAPTER VII. 



Technique of Isolating Bacteria in Pure Culture by the Plate and 



the Tube Method . . . . . . . . . ... . 137 



CHAPTER VIII. 



The Incubating Oven The Safety Burner Employed in Heating 



the Incubator Thermo-regulator Gas-pressure Regulator 145 



CHAPTER IX. 



The Study of Colonies Their Naked-eye Peculiarities and Their 

 Appearance Under Different Conditions Differences in the 

 Structure of Colonies from Different Species of Bacteria 

 Stab-cultures Slant-cultures . . .. . k . . . . 152 



CHAPTER X. 



Methods of Staining Cover-slip Preparations Impression 

 Cover-slip Preparations Solutions Employed Preparation 

 and Staining of Cover-slips Staining Solutions Special 

 Staining Methods 158 



CHAPTER XI. 



Systematic Study of an Organism Points to be Considered in 

 Determining the Morphologic and Biologic Characters of a 

 Culture Methods by which the Various Biologic and Chem- 

 ical Characters of a Culture may be Ascertained Dark 

 Field Illumination Facts Necessary to Permit the Identifi- 

 cation of an Organism as a Definite Species 179 



