150 BACTERIOLOGY 



and as cooling begins a gradual contraction of the mercury 

 occurs until there is again an outflow of gas from the opening 

 h, when the temperature again rises. This contraction and 

 expansion of the mercury in the regulator continues until 

 eventually a point is reached at which its position in the 

 cylinder e allows of the passage of just enough gas from the 

 opening h to maintain a constant temperature and, there- 

 fore, a constant degree of expansion of the mercury in the 

 tube e. This, in short, is the principle on which thermo- 

 regulators are constructed; but it must be borne in mind 

 that a great deal of detail exists in the construction of an 

 accurate instrument. The number of different forms of 

 this apparatus is comparatively large, and each form has 

 its special merits. 



The value that is, the delicacy of the thermo-regulator 

 depends upon a number of factors, all of which it would be 

 useless to describe in a book of this kind; but in general 

 it may be said that the essential points to be observed in 

 selecting a thermo-regulator depend in the main upon 

 the temperatures at which it is to be used. For low tem- 

 peratures, regulators containing such fluids as ether, alcohol, 

 and calcium chloride solution, which expand and contract 

 rapidly and regularly under slight variations in temperature, 

 are commonly employed; whereas for temperatures approach- 

 ing the boiling-point of water mercury is most frequently used. 



The temperature of the incubator is to be regulated, then, 

 by the use of some such form of apparatus as that just 

 described. It should be of sufficient delicacy to prevent 

 a fluctuation of more than 0.2 C. in the temperature of the 

 air within the chamber of the apparatus. 



Gas-pressure Regulators. A gas-pressure regulator is not 

 rarely intervened between the gas-supply and the thermo- 



