220 BACTERIOLOGY 



rectal temperature of these small animals is to be taken. 

 The manipulation can easily be made without the aid of 

 an assistant. Its construction is seen in Fig. 42. l 



For ordinary subcutaneous inoculations at the root of the 

 tail in mice a simple apparatus consists of a piece of board 

 about 7 x 10 cm. and 2 cm. thick, upon which is tacked a 

 hollow truncated cone of wire gauze, about 6 cm. long and 

 about 1.5 cm. in diameter at one end and 2 cm. at its other 

 end. This is tacked upon the board in such a position that 

 its long axis is in the long axis of the board, being equidistant 

 from its sides. Its small end is placed at the edge of the 



FIG. 43 



Mouse-holder, with mouse in proper position. 



board. The mouse is taken up by the tail by means of a 

 pair of tongs and allowed to crawl into the smaller end of 

 the wire cone. When so far in that only the root of the 

 tail projects the animal is fixed in this position by a clamp 

 and thumb-screw, with which the apparatus (Fig. 43) is 

 provided. The animal usually remains perfectly quiet and 

 may be handled without difficulty. 



The hair over the root of the tail is,to be carefully cut 

 away with scissors and a pocket cut through the skin at 

 this point. The inoculation is then made into the loose 



1 Centralblatt fur Bacteriologie and Parasitenkunde, 1895, vol. xviii, p. 530. 



