364 APPLICATION OF METHODS OF BACTERIOLOGY 



NOTE. To a fresh agar culture of this organism, in 

 which the green coloration of the medium is especially 

 marked, add about 2 c.c. of chloroform. Shake gently, and 

 note that the chloroform extracts a blue coloring-matter 

 from the culture, leaving the latter more or less yellow. 



FIG. 70 



FIG. 71 



FIG. 72 



FIG. 70. Stab-culture of ps. ceruginosa in gelatin after twenty-eight 

 hours at 22 C. 



FIG. 71. Colony of ps ceruginosa after twenty-four hours on gelatin at 

 20-22 C. 



FIG. 72. Colony of ps. ceruginosa after forty-two hours on gelatin at 

 20-22 C. 



Allow the chloroform extract to stand for several days; 

 note what occurs; how do you account for it? 



Prepare a 100 c.c. Ehrlenmeyer flask with 75 c.c. of 

 sterile bouillon or peptone solution. Inoculate it with this 



