BACTERIUM MALLEI 451 



for thirty minutes; absorb all superfluous stain with blot- 

 ting-paper, and wash the section three times with 0.3 per 

 cent, acetic acid, not allowing the acid to act for more than 

 ten seconds each time. Remove all acid from the section 

 by carefully washing in distilled water; absorb all water 

 by gentle pressure with blotting-paper; and finally, at very 

 moderate heat, or with a small bellows (Kiihne), dry the section 

 completely on the slide. When dried clear in xylol and mount 

 in xylol balsam. 



b. Transfer the sections from alcohol to distilled water; 

 from water to the dilute fuchsin solution, and gently warm 

 (about 50 C.) for fifteen to twenty minutes. Transfer 

 sections from the staining-solution to the slide, absorb all 

 superfluous stain with blotting-paper, and then treat them 

 with 1 per cent, acetic acid from one-half to three-quarters 

 of a minute. Remove all trace of acid with distilled water, 

 absorb all water by gentle pressure with blotting-paper, and 

 then treat the sections with absolute alcohol by allowing 

 it to flow over them drop by drop. For small sections three 

 or four drops are sufficient. Under no circumstances should 

 the alcohol be allowed to act for more than one-quarter of 

 a minute. Clear in xylol and mount in xylol balsam. 



By method 6 the tissues are better preserved than by 

 method a, by which they are dried. 



In properly stained tissues the bacteria will be found 

 most numerous in the centre of the nodules, becoming fewer 

 as we approach the periphery. They usually lie between 

 the cells, but at times may be seen almost filling some of 

 the epithelial cells, of which the nodule contains more or less. 

 They are always present in these nodules in the tissues; they 

 are rarely present in the blood, and, if so, in only small 

 numbers. 



