572 APPLICATION OF METHODS OF BACTERIOLOGY 



animals as the organisms growing in the flasks? Is the 

 action of each of these cultures the same for mice, guinea- 

 pigs, and rabbits? 



Prepare a 2 per cent, solution of sulphuric acid in dis- 

 tilled water; suspend in this a number of anthrax spores; 

 at the end of three, six, and nine days at 35 C. inoculate 

 both a guinea-pig and a rabbit. Prepare cultures from this 

 suspension on the third, sixth, and ninth days; when the 

 cultures have developed inoculate a rabbit and a guinea- 

 pig from the culture made on the ninth day. Should the 

 animals survive, inoculate them again after three or four 

 days with a culture made on the sixth day. Do the results 

 appear in any way peculiar? 



