590 APPLICATION OF METHODS OF BACTERIOLOGY 



media, but not with access of oxygen. It grows well in an 



atmosphere of hydrogen. It causes liquefaction of gelatin. 



In tubes containing about 20 to 30 c.c. of gelatin that 



has been liquefied, inoculated with a small amount of the 



FIG. 102 



Bacillus edematis. A, edema-fluid, from site of inoculation of guinea-pig, 

 showing long and short threads; B, spore-formation, from culture. 



culture, and then rapidly solidified in ice-water, growth 

 appears in the form of isolated colonies at or near the bottom 

 of the tube in from two to three days at 20 C. These 

 colonies, when of from 0.5 to 1 mm. in diameter, appear as 

 spheres filled with clear liquid, and are difficult, for this 



