x CONTENTS. 



CHAPTER VI. 



PAGE 



Preparation of the tubes, flasks, etc., in which the media are to 

 be preserved 104-107 



CHAPTEE VII. 



Technique of making plates Esmarch tubes, Petri plates, etc. . 108-119 



CHAPTEE VIII. 



The incubating oven Gas-pressure regulator Thermo-regulator 

 Safety burner employed in heating the incubator .... 120-127 



CHAPTEE IX. 



The study of colonies Their naked-eye peculiarities and their ap- 

 pearance under different conditions Differences in the structure 

 of colonies of different species of bacteria Stab cultures Slant 

 cultures . . . ; . . . ..... .' . . 128-132 



CHAPTEE X. 



Methods of staining Solutions employed Preparation and stain- 

 ing of cover-slipsPreparation of tissues for section-cutting Stain- 

 ing of tissues Special staining methods 133-169 



CHAPTEE XI. 



Systematic study of an organism Points to be considered in iden- 

 tifying an organism as a definite species . . . , . . 170-196 



CHAPTEE XII. 



Inoculation of animals Subcutaneous inoculation ; intravenous 

 injection Inoculation into the great serous cavities ; and into the an- 

 terior chamber of the eye Observation of animals after inoculation 197-218 



CHAPTEE XIII. 



Post-mortem examination of animals Bacteriological examina- 

 tion of the tissues Disposal of tissues and disinfection of instru- 

 ments after the examination . . . 219-224 



