CONTENTS. 



INTRODUCTION. 



The overthrow of the doctrine of spontaneous generation" Omne 

 vivum ex vivo " Earlier bacteriological studies The birth of mod- 

 ern bacteriology 13-2G 



CHAPTEK I. 



Definition of bacteria Their place in nature Difference between 

 parasites and saprophytes Nutrition of bacteria Products of bac- 

 teriaTheir relation to oxygen Influence of temperature upon 

 their growth 27-35 



CHAPTEE II. 



Morphology of bacteria Grouping Mode of multiplication 

 Spore-formation-Motility . 36-46 



CHAPTER III. 



Principles of sterilization by heat Methods employed Discon- 

 tinued sterilization Sterilization under pressure Apparatus em- 

 ployedChemical disinfection and sterilization . . . . . 47-70 



CHAPTER IV. 



Principles involved in the methods of isolation of bacteria in pure 

 culture by the plate method of Koch Materials employed . . 71-76 



CHAPTER V. 



Preparation of nutrient media Bouillon, gelatin, agar-agar, potato, 

 blood-serum, etc * 77-103 



