188 



BACTERIOLOGY. 



tubes of Liborius : The tube is first inoculated as 

 if it were to be poured as a plate or rolled as an ordi- 

 nary Esmarch tube. The cotton plug is then re- 

 placed by a rubber stopper, through which pass two 

 glass tubes. These must all have been sterilized in the 

 steam sterilizer before using. On the outer side of the 

 stopper these two tubes are bent at right angles to the 



FIG. 36. 



Frankel's method for the cultivation of anaerobic bacteria. 



long axis of the test-tube into which they are to be 

 placed, and both are slightly drawn out in the gas 

 flame. At the outer extremity of both of these tubes 

 a plug of cotton is placed ; this is to prevent access of 

 foreign organisms during manipulation. At the inner 

 side of the rubber stopper that is, the end which is to 



