196 BACTERIOLOGY. 



the media both in media containing fermentable sugars 

 and those from which these bodies are absent? When 

 cultivated in sugar-bouillon in the fermentation tube, 

 what production of gas is evolved under known con- 

 ditions ? How much of this gas is carbonic acid and 

 how much is explosive? 



12. At what temperature does it thrive best, and the 

 lowest and highest temperatures at which it will de- 

 velop ? What is its thermal death-point, both by steam 

 and dry-air methods of determining this point? 



Ie3. What is its behavior when exposed to chemical 

 disinfectants and antiseptics ? Does it withstand drying 

 and other injurious influences, both in the vegetative and 

 spore stages ? The germicidal value of the blood-serum 

 of different animals may also be tried upon it. 



14. Its pathogenic powers modes of inoculation by 

 which these are demonstrated ; quantity of material used 

 in inoculation ; duration of the disease and its symptoms; 

 lesions produced, and distribution of the bacteria in the 

 inoculated animal ; which animals are susceptible and 

 which immune, and the character of its pathogenic activ- 

 ities? Variations in virulence, and the probable cause 

 to which they are due. Can they be produced artificially 

 and at will ? 



15. The detection of specific, toxic, and immunizing 

 products of growth. 



