200 BACTERIOLOGY. 



tudinal slit of 12 mm. wide that runs for 9.5 cm. 

 from within 0.5 mm. of the open extremity of the cylin- 

 der. 



The animal is placed in such a cylinder with its head 



FIG. 38. 



Holder for larger animals. 



toward the perforated bottom. It is then easily possible 

 to make subcutaneous inoculation by taking up a bit of 

 skin through the slit in the side of the box, or to make 

 intra-peritoneal injection by drawing the posterior ex- 

 tremities slightly from the box and holding them steady 

 between the index and second finger, as seen in Fig. 39. 

 It is also very convenient for use when the rectal tem- 

 perature of these small animals is to be taken. The 

 manipulations can easily be made without the aid of an 

 assistant. Its construction is best seen in Fig. 39. 



For ordinary subcutaneous inoculations at the root 

 of the tail in mice a simple piece of apparatus consists 

 of a bit of board of about 7 x 10 cm. and 2 cm. thick, 

 upon which is tacked a hollow, tapering roll of wire 

 gauze, a truncated cone, of about 6 cm. long and of 

 about 1.5 cm. in diameter at one end and 2 cm. at its 

 other end. 



