ANIMALS AFTER INOCULATION. 21 1 



plications. All instruments, sutures, ligatures, etc., 

 must be carefully sterilized either in the steam sterilizer 

 for twenty minutes, or by boiling in 2 per cent, sodium 

 carbonate solution for ten minutes ; the hands of the oper- 

 ator, though they should not touch the wound, should 

 be carefully cleansed and the material to be introduced 

 into the abdomen should be handled with only sterilized 

 instruments. 



Inoculation into the pleural cavity is much less fre- 

 quently called for in fact, it is not a routine method 

 employed in this work. It is not easy to enter the 

 pleural cavity with a hypodermic needle without injur- 

 ing the lung, and it is rare that conditions call for the 

 introduction of solid particles in this locality. 



Inoculation into the anterior chamber of the eye is per- 

 formed by making a puncture through the cornea just 

 in front of its junction with the sclerotic, the knife being 

 passed into the anterior chamber in a plane parallel 

 to the plane of the iris. By the aid of a fine pair of 

 forceps the bit of tissue is passed through the opening 

 thus made and is deposited upon the iris, where it is 

 allowed to remain, and where its pathogenic properties 

 upon the iris can be conveniently studied. It is a mode 

 of inoculation of very limited application, and is there- 

 fore but rarely practised. It was employed by Cohnheim 

 in demonstrating the infectious nature of tuberculous 

 tissues, tuberculosis of the iris being the constant result 

 of the introduction of tuberculous tissue into the 

 anterior chamber of the eye of rabbits. 



OBSERVATION OF ANIMALS AFTER INOCULATION. 



After either of these methods of inoculation, particu- 

 larly when unknown species of bacteria are being tested, 



