THE BACILLUS OF GLANDERS. 291 



at the end of from twenty-four to thirty-six hours at 

 37 C. as a moist, amber-yellow, transparent deposit 

 which becomes deeper in color and denser in consistence 

 as growth progresses. It finally takes on a reddish- 

 brown color, and the potato about it becomes darkened. 



In bouillon it causes diffuse cloudiog, with ultimately 

 the formation of a more or less tenacious or ropy sedi- 

 ment. 



In milk to which a little litmus has been added, it 

 causes the blue color to become red or reddish in from 

 four to five days, and quite red after two weeks at 37 

 C. At the same time the milk is separated into a firm 

 clot of casein and clear whey. 



Its reactions to heat are very interesting at 42 C. 

 it will often grow for twenty days or more. It will not 

 grow at 43 C., and is killed by exposure to this tem- 

 perature for forty-eight hours. It is killed in five hours 

 when exposed to 50 C., and in five minutes by 55 C. 



It grows both with and without oxygen ; it is there- 

 fore facultative as regards its relation to this gas. 



On cover-slips it stains readily with all the basic 

 aniline dyes, and, as a rule, as stated, presents conspicu- 

 ous irregularities in the way that it takes up the dyes, 

 being usually marked by deeply stained areas that 

 alternate with points at which it either does not stain 

 at all or only slightly. 



The animals that are susceptible to infection by this 

 organism are horses, asses, field mice, guinea-pigs, and 

 cats. Baumgarten records cases of infection in lions 

 and tigers that have been fed, in menageries, with flesh 

 from horses affected with the disease. Rabbits are but 

 slightly susceptible ; dogs and sheep still less so. Man 

 is susceptible, and infection not rarely terminates fatally. 



