306 BACTERIOLOGY. 



larger. They are white, and do not project above the 

 surface of the capsule. 



The bacilli are always to be found at the seat of in- 

 oculation, most abundant in the grayish-white, fibrino- 

 purulent exudate. They become fewer at a distance from 

 this, so that the more remote parts of the oedematous 

 tissues do not contain them. They are found not only 

 free, but contained in large number in leucocytes, some 

 of which have fragmented nuclei, or have lost their 

 nuclei. The bacilli within leucocytes, as well as some 

 outside, frequently stain very faintly and irregularly, 

 and may appear disintegrated and dead. 



Culture-tubes inoculated from the blood, spleen, liver, 

 kidneys, ad-renal bodies, distant lymphatic glands, and 

 serous transudates, generally yield negative results ; and 

 negative results are also obtained when these organs are 

 examined microscopically for the bacilli. 



Microscopic examination of the tissues at the seat of 

 inoculation, as well as of the liver, spleen, kidneys, 

 lymphatic glands and elsewhere, reveals the presence 

 of localized foci of cell death, characterized by a peculiar 

 fragmentation of the nuclei of the cells of these parts. 



This destruction of nuclei results in the occurrence 

 of groups of irregularly shaped, deeply staining bodies, 

 having at some times the appearance of particles of 

 dust, while again they may be much larger. Some of 

 them are tolerably regular in outline, while others are 

 irregularly crescentic, dumb-bell, flask-shape, whetstone- 

 shape, or bladder-like in form. Occasionally nuclei 

 having the appearance of being pinched or drawn out 

 can be seen. At some points the fragments are grouped 

 into isolated masses, indicating the location of the nucleus 

 from the destruction of which they originated. These 



