364 BACTERIOLOGY. 



method employed by Koch in his cholera experiments, 

 Finkler and Prior had 3 out of 10 animals, and Koch 5 

 out of 15 animals so treated to die. 



The claim of Finkler and Prior that this organism 

 was related etiologically to cholera nostras has been 

 shown by subsequent work to have been unjustifiable. 



In 1885, 1886, and 1887, Franck 1 examined seven 

 cases that clinically presented the condition of cholera 

 nostras ; in none of these seven cases was the organism 

 of Finkler and Prior, which they claimed to be the 

 cause of the disease, found. In all cases the results of 

 bacteriological examination, in so far as the constant 

 presence of an organism that might stand in causal re- 

 lation to the disease was concerned, were negative. Only 

 the ordinary intestinal bacteria were found. 



SPIEILLUM TYROGENUM (CHEESE SPIRILLUM OF 

 DENEKE). 



Another spiral form, likewise forming short, comma- 

 shaped segments in the course of its growth (Fig. 71), is 

 that found by Deneke in old cheese. It is a little smaller 

 than Koch's spirillum. It is motile and has but a single 

 flagellum, attached to one of its ends. It liquefies gelatin 

 more rapidly than does Koch's organism. It possesses no 

 characteristic grouping, as can be seen in impression cover- 

 slips of its colonies. It does not form spores. On gelatin 

 plates its colonies develop very rapidly as saucer-shaped 

 depressions ; after twenty-four hours they vary from 1 to 

 4 mm. in transverse diameter. To the naked eye they 

 are almost transparent, and are usually marked by a 



i Zeitschrift f. Hygiene, Bd. iv. p. 207. 



