478 BACTERIOLOGY. 



Glass handles to be of about 3 mm. thickness and from 

 15 to 17 cm. long. 



STAINING AND MOUNTING REAGENTS. 



200 c.c. of saturated alcoholic solution of fuchsiu. 



200 c.c. of saturated alcoholic solution of gentian 

 violet. 



200 c.c. of saturated alcoholic solution of methylene- 

 blue. 



200 grammes of pure aniline. 



200 grammes of C. P. carbolic acid. 



500 grammes of C. P. nitric acid. 



500 grammes of C. P. sulphuric acid. 



200 grammes of C. P. glacial acetic acid. 



1 litre of ordinary 93-95 per cent, alcohol. 



1 litre of absolute alcohol. 

 500 grammes of ether. 



500 grammes of pure xylol. 

 50 grammes of Canada balsam dissolved in xylol. 

 100 grammes of Schering's celloidin. 

 10 grammes of iodine and 30 grammes of iodide of 

 potassium in substance. 

 100 grammes of tannic acid. 

 100 grammes of ferrous sulphate. 

 Distilled water. 



FOR NUTRIENT MEDIA. 



J- pound Liebig's or Armour's beef extract. 

 250 grammes Witte's peptone. 



2 kilogrammes of gold label gelatin (Hesteberg's). 

 100 grammes of agar-agar in substance. 



