vi CONTENTS 



CHAPTER V. 



Reactions, Methods for Adjustment Titration Hydrogen ion 

 Concentration Preparation of Media Bouillon, Gelatin, 

 Agar-agar, Potato, Blood Serum, Blood Serum from Small 

 Animals, Milk, Litmus-whey Milk, Dm ham's Peptone Solu- 

 tion, Lactose Litmus-agar, Loffler's Blood Serum Mixture, 

 the Serum Water Media of Hiss, Guarniari's Gelatin-agar 

 Mixture Ill 



CHAPTER VI. 



Preparation of the Tubes, Flasks, etc., in which the Media are to 



be Preserved 145 



CHAPTER VII. 

 Technique of Isolating Bacteria in Pure Culture by the Plate and 



the Tube Method 149 



CHAPTER VIII. 



The Incubating Oven The Safety Burner Employed in Heating 



the Incubator Thermo-regulator Gas-pressure Regulator 157 



CHAPTER IX. 



The Study of Colonies Their Naked-eye Peculiarities and Their 

 Appearance Under Different Conditions Differences in the 

 Structure of Colonies from Different Species of Bacteria 

 Stab Cultures Slant Cultures 165 



CHAPTER X. 



Methods of Staining Cover-slip Preparations Impression 

 Cover-slip Preparations Solutions Employed Preparation 

 and Staining of Cover-slips Staining Solutions Special 

 Staining Methods 171 



CHAPTER XL 



Systematic Study of an Organism Points to be Considered in 

 Determining the Morphologic and Biologic Characters of a 

 Culture Methods by which the Various Biologic and Chem- 

 ical Characters of a Culture may be Ascertained Variation 

 and Varieties Dark Field Illumination Facts Necessary 

 to Permit the Identification of an Organism as a Definite 

 Species 191 



