374 APPLICATION OF METHODS OF BACTERIOLOGY 



By the use of the above mediflm he has cultivated the gono- 

 coccus from about one hundred different cases. 



If micrococcus gonorrhceae be transplanted from the origi- 

 nal culture to either glycerin-agar-agar or to Loffler's serum 

 mixture, a growth is sometimes observed, more often in the 

 latter than in the former, but of so feeble a nature that these 

 substances cannot be regarded as suitable for its cultivation 

 and certainly not for its direct isolation from the body. As 

 a rule, development does not occur on glycerin-agar. 



Its growth is favored by at least partial anaerobic con- 

 ditions. 



Microscopic examination of colonies of this organism 

 reveals the presence of a diplococcus somewhat larger than 

 the ordinary pyogenic cocci. The opposed surfaces of the 

 individual cells that comprise the couplets are flattened and 

 separated by a narrow slit. At times the cocci are arranged 

 as tetrads. 



This organism cannot be grown at a temperature lower 

 than that of the human body, and cultures that have been 

 obtained by either of the favorable methods are said to 

 lose their vitality when kept at ordinary room-temperature 

 for about two days. 



It is killed in a few hours by drying. 



Cultures retain their vitality under favorable conditions 

 of nutrition, temperature, and moisture for from three to 

 four weeks. 



This organism is without pathogenic properties for 

 monkeys, dogs, and horses, as well as for the ordinary 

 smaller animals used for this purpose in the laboratory. 



In man typical gonorrhea has been produced by the 

 introduction into the urethra of pure cultures of this 

 organism. 



