380 APPLICATION OF METHODS OF 'BACTERIOLOGY 



Its growth in bouillon is slow and uncertain. It does not 

 cause clouding of the fluid, but collects at the bottom of the 

 tube as a scanty grayish sediment, that when disturbed 

 gives the impression of having a mucoid consistency. ;> . 



It does not grow on potato and causes no change in litmus- 

 ' milk. 



It grows only at the temperature of the body, and can 

 be kept growing only by being transplanted to fresh media 

 about every two days, and even then growth often ceases 

 after a comparatively small number of transplantations. 

 If from a fresh growing culture a number of tubes be inocu- 

 lated and kept under favorable conditions it is a common 

 experience to have growth on only a part of them. It is 

 sometimes impossible to obtain a second growth on agar-agar. 



In addition to its presence in the meningeal exudation 

 of epidemic cerebrospinal meningitis, this organism may 

 appear as a secondary invader of the lung, causing more or 

 less extensive pneumonic exudation; of the joints; the ear; 

 the eye; and the nose and throat. Though rarely, its 

 presence in the circulating blood may sometimes be demon- 

 strated. 



Subcutaneous inoculation with pure cultures has usually 

 no effect. Injections into the great serous cavities may or 

 may not result in serofibrinous or fibrinopurulent inflam- 

 mation. Positive results are oftener obtained on young 

 guinea-pigs weighing about 150 grams, than on larger, 

 more mature animals. Intravenous inoculations are equally 

 unsatisfactory, though the results depend upon the original 

 virulence, the age of the culture and the animal selected. 

 In horses toxic symptoms are often the conspicuous result of 

 this mode of inoculation. 



The only successful attempts to reproduce the morbid 



