576 APPLICATION OF METHODS OF BACTERIOLOGY 



microscope, show a yellowish-brown, ragged central mass 

 surrounded by a zone of liquefaction that is marked by a 

 border of delicate radii. (Fig. 93.) 

 In gelatin stab-cultures the growth has much the same 



FIG. 94 



abed 

 Stab-culture of microspira Metchnikovi in gelatin, at 18 to 20 C. a, 

 after twenty-four hours; 6, after forty-eight hours; c, after seventy-two 

 hours; d, after ninety-six hours. 



general appearance as that of the cholera spirillum, but is 

 exaggerated in degree. The liquefaction is far more rapid, 

 and the characteristic appearance of the growth is lost in 

 from three to four days. (See a, b, c, d, Fig. 94.) Develop- 

 ment and liquefaction along the deeper parts of the needle- 



