210 THE HYDROLYZING ENZYMES 



Saliva-Diastase on Starch (A. E. Taylor) ; a = 0.25 per cent. Starch: 



10K = logic 



Time in minutes. 



t a x 



30 . . . '; . . . .'* . .- . . , 490 



45 465 



60 455 



75 470 



90 465 



120 .455 



150 ; v 460 



180 . . : . ..... . , 455 



Trypsin on d-alanyl-d-alanine (Abderhalden and Koelker) : 



103 X 



K = - 



Time in minutes. t 



50 ' v 380 



65 .400 



7.5 . 400 



16.0 369 



22.0 368 



28.0 368 



30.0 ,-.-.' 314 



38.0 ; 332 



Trypsin on Sodium Caseinate (E. H. Walters) : 



10* a 



104 K = logio 



Time in minutes. t a x 



15 18. 



30 16. 



45 : 15. 



60 -....,,...., 14. 



75 13. 



90 ..... ^ ........... 12.5 



105 12. 



120 . 12. 



135 12. 



150 13. 



165 .13. 



180 12.5 



210 ................. 13. 



240 '..'- 12.5 



270 . . \ ;..... .-..:.- Y ... ... ... 12. 



300 .. . . . . . . . . . . . . :. . . . 13.5 



330 ' . . 13. 



360 . . . . . ... . . . . . ... . .14. 



420 . \ .'.-. V . . . 14.5 



480 ;' . . . . . ". . '. " '.. . 14. 



540 . . ... .... . . . 14. 



In certain instances yet another type of relationship between the 

 amount of transformation and the time may be observed. In the 

 clotting of milk by Rennin and in the hydrolysis of proteins by Pepsin 

 we find that the mass "x" of the substance transformed is connected 

 with the time "t," the initial concentration of the substrate "a," and 

 the concentration of the enzyme "F" by the relationship: 



k\Art 



