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Biological Therapy 



PITMAN-MOORE TUBERCULINS 



Numerous strains of the tubercle bacilli which are 

 known to be exceptionally desirable for tuberculin produc- 

 tion are used for this purpose. These strains are grown on 

 the surface of glycerine bouillon for a period of approxi- 

 mately twelve weeks, during which time they eliminate an 

 effete end product which saturates the media. When the 

 media is fully saturated or charged the tubercle bacilli are 

 killed by subjecting the flasks and their contents to steriliza- 

 tion in an autoclave. The content of the various flasks is 

 now placed on a water bath and evaporated to one-tenth 

 of its original volume. After this evaporation the product 

 is filtered in such manner that all bacteria are removed and 

 there remains a sterile product incapable of causing disease. 

 The product concentrated in this manner is known as Koch's 

 Old Tuberculin. Beginning with Koch's Old Tuberculin as a 

 basis or concentrate the various kinds of tuberculins are 

 prepared. 



PITMAN-MOORE SUBCUTANEOUS TUBERCULIN is 

 prepared by adding to the concentrate described above a 

 definite amount of glycerine and normal saline solution. The 

 Bureau of Animal Industry requires that each cattle dose 

 of subcutaneous tuberculin shall contain .5 grams of Koch's 

 Old Tuberculin. Pitman-Moore subcutaneous tuberculin 

 contains .6 grams of Koch's Old Tuberculin, thus insuring 

 an ample amount of tuberculin to insure dependable re- 

 actions. 



PITMAN-MOORE INTRADERMAL TUBERCULIN. In 

 this, as in the case of subcutaneous tuberculin, the start is 

 made with the concentrate or Koch's Old Tuberculin. Enough 

 saline is added so that each cubic centimeter contains .5 

 grams of Koch's Old Tuberculin. This product is prepared 

 in such a manner that it minimizes the possibility of reac- 

 tions occurring from causes other than tuberculosis. 



PITMAN-MOORE TRIPLE STRENGTH TUBERCULIN 

 is prepared in the same general manner as subcutaneous 

 tuberculin and contains 1.8 grams of Koch's Old Tuberculin 

 to each cattle dose. This product is admirably suited for the 

 subcutaneous retesting of cattle and for the testing of cattle 

 suspected of having been "plugged." The dose is four c.c. 



