17 



distiiied water in from 3-4 hours and produce long filamente 

 in eighteen hours, a feature which is of considerable advan- 

 tage here, for it is quite conceivable the influence of var- 

 ious chemical compounds on each other and on the germinating 

 spores may be considerably altered by the presence of nut- 

 rient salts in the solution. In view of these considerations 

 these experiments were carried out without the use of nutri- 

 ent media. 



