2'6 



EXP3RIM3NTATI0N 

 In these studies any renewed activity in the proto- 

 plasm of the spore was considered as germination. Several 

 forms of such renewed activity may be exhibited in these 

 spores. Without any alteration in size or shape, a portion 

 of the spore may become nearly or quite opaque, thus appear- 

 ing dark brown or black by transmitted light. A papilla may 

 be formed at any point on the surface. Such papillae may or 

 may not enlarge to form rounded bodies and may either remain 

 hyaline or become apparently darkened. Papillae may enlarge 

 into irregular shapes or may extend outward as markedly 

 thickened tubes. Lastly, the growing papillae may take the 

 form of slender tubes. The latter type of germination always 

 occurs in distilled water and in a period of 18 hours the 

 tubes attain a length at least twice as great as that of the 

 spore itself.. In the following treatment germination will 

 be considered "normal" wherever tubes of the last mentioned 

 type, at least twice the length of the spore, were produc- 

 ed in 18 hours. 



In the present section will be considered the vari- 

 ous effects upon germination brought about in the presence 

 of the following salts either alone or in certain combinat- 

 ions:- 



CaCNOj)^ Pb(K03)2 Ni(N03)2 

 IvIg{N03)2 Zn(N03)2 Cu(N03)2 

 KNO3 A1(N03)3 CuSG^ 



