43 



appressoria. Some of the similar hyaline swellings also 

 occurred in these cultures. '.Vhen cultures where appressoria 

 were common were allowed to remain in the thermostat for 

 several days and were examined from time to time, these 

 swellings continued to develop successively from the same 

 spore, until sometimes as many as four appeared together 

 at one end (see fig. 4 ). In the formation of chains of 

 these bodies it appeared from the observation of different 

 stages thatla second or later swelling may be brought about 

 either by the germination of one previously formed or by 

 enlargement of the constricted portion of the tube between 

 one previously formed and the spore itself, in the latter 

 case possibly by new growth from the spore itself. In many 

 cases a light colored area quite like that considered by 

 Hasselbring to be a pore, could be distinguished and it may 

 have bean present in all cases. 



Spores which had germinated in drop cultures by 

 forming appressoria were transferred to new cultures in 

 distilled water and returned to the thermostat. Nearly all 

 the appressoria had thrust out normal germ tubes at the 

 end of 24 hours. 



An occasional spore germinated normally in a con- 

 centration of C. 00005m Pb(N03)2, and a few normal tubes 

 were found in 0.0C0G4m concentration, but it was only in 

 the 0.00002 -concentration that no effect of the lead nitrat-3 

 upon the germination of the spores was noticeable. In this 



