5i) 



in Stigeoclonium . The third type of response discussed by 

 Livingston, change in phenomena of reproduction, of course 

 finds no parallel in this investigation. 



For convenience of comparison , the limits of the 

 various responses of the fungus spores here dealt with, to 

 the different treatments employed, have been presented in 

 the form of a table and are given below. The only nitrate 

 occurring in the table that has not already ^recieved attent- 

 ion is the acid. In a series of cultures with HKC3 the ger- 

 mination at a C.0C5m concentration was practically all nor- 

 mal while at 0.01m and all higher concentrations no germin- 

 ation took place in the 18 hour period. The acid prevented 

 germination for the period of 18 hours at a 0.02° concen- 

 tration and killed the spores at Com. No concentration was 

 found, therefore, at which the germination tok the form of 

 any of the various abnormal growths found at some concentra- 

 tion with all the other substances used, nor was there any 

 apparent coagulation of the protoplasm at concentrations 

 below that which killed the spore. From these considerations 

 it would seem then that the various abnormal growths and 

 effects on the protoplasm of the spore cannot be due to the 

 acid present in the solution as a result of hydrolysis of 

 the salts, but must be related either directly or indirectly 

 to the metals themselves. 



Turning now to the tables, the different substances 

 are there arranged in the order of the concentration which 



