RABIES 301 



(2) The Intraocular Inoculation (Gibier, Nocard, Johne). — The 



emulsion (1 to 2 dropt^) wliit-h is thus obtained is filtered through a 

 piece of linen antl injected directly into the anterior chamber of the eye 

 of the animal which is to bo inoculated. They do this by means of a 

 small hypodermic syringe, having first placed cocaine on the cornea, 

 and then inject the solution directly into the anterior chamber. If the 

 suspected animal is raliid, we will see the development of the disease in 

 from twelve to twenty-three days, even if the chaml)er should suppurate 

 from the irritation of the injected solution. Gal and Klimmer oppose 

 this procedure by pointing out the fact that the stage of incubation 

 may be much longer than this. 



(3) The Intraspinal Method (Labell). — The emulsion is injected 

 directly in the cord; this method takes somewhat longer to develop than 

 the subdural inoculation. 



(4) Intramuscular Inoculation. — One c.c. of the emulsion is 

 injected into the masseter, the dorsal or the posterior crural. Klimmer 

 finds that the active symptoms are developed somewhat earlier than by 

 the intraocular method. 



There are other methods of inoculation used l3ut they are much 

 less reliable, such as nasal, subconjunctival, subcutaneous and intravenous. 



It is always w^ell to inoculate two animals, because it frequently 

 happens that an animal is immune to the disease or dies shortly after 

 the inoculation (cerebral hemorrhage, etc.). 



The following diseases are sometimes mistaken for rabies: Certain 

 affections of the brain, such as teething, epilepsy, eclampsia, distemper, 

 angina, intestinal parasites, inflammation of the intestines, pentastoma 

 in the nose and frontal cavities, foreign bodies in the mouth (between 

 the teeth) or in the throat, paralysis of the lower jaw, luxation of the 

 lower jaw, due to irritation of the tiigeminus, intense excitement in 

 latches that have had their young taken from them, great sexual excite- 

 ment in male dogs, long confinement in cages or kennels, and from 

 certain poisons. The course of the disease, however, and the after- 

 symptoms always enal^le one to make a differential diagnosis. 



Therapy and Prophylaxis. — As soon as the disease has reached a 

 point where there is no question as to its character, the animal should 

 be destroyed as soon as possible. When man is bitten it is a question 

 whether thorough disinfection or cauterization of the wound is of much 

 benefit unless it is done within a few minutes. In reference to the pre- 

 ventive inoculation of Pasteur, it is not necessary to enter into detail in 

 this work, beyond the fact that it is the inoculation of an attenuated virus 

 cultivated from rabies. A large number of experimenters have made 

 repeated inoculations with a view of obtaining immunity to the disease, 

 but they have not had very satisfactory results. 



