11 



When the inoculated material contains large numbers of other 

 bacteria, as is often the case, intraperitoneal inoculation may result 

 in death from peritonitis. In such cases it is best to inoculate 

 two guinea pigs, one intraperitoneally and the other subcutaneously. 

 In subcutaneous inoculation local ulceration is usually followed in 

 a few days by involvement of the testicles or other organs. 



Nocard has shown that this test is not altogether infallible, 

 as a bacillus is found in another disease of horses, characterized 

 by ulceration of the extremities, that produces lesions, closely 

 resembling those produced by Bacterium mallei, in the testicles of 

 guinea pigs. 



Frothingham, in reviewing his extensive experience with the 

 method of Strauss, had only four absolute failures out of a large 

 number of tests. His statistics show that, where in a few instances 

 no scrotal lesions are produced in the guinea pig, glanders abscesses 

 may occur in other parts of the body. In discussing the fallacies 

 of the test he mentions the bacillus found by Kutscher in the nasal 

 secretion of a glandered horse which closely resembles Bacterium 

 mallei in its morphology and produces similar lesions in the testicles 

 of guinea pigs. However, the former organism yields a white 

 growth on potato, which is easily distinguished from the amber- 

 colored, honey-like growth of the bacterium of glanders. The Ba- 

 cillus pyocyaneus may also produce a somewhat similar growth on 

 potato, but is easily distinguished by its motility in the hanging 

 drop. He emphasizes the fact that in the use of the Strauss 

 method a positive result means everything, while a negative one 

 means nothing. 



(2) By the injection of mallein.' 1 Mallein is prepared, after 

 the manner of tuberculin, by growing a freshly isolated culture of 

 Bacterium mallei in glycerine bouillon for from four to six weeks. 

 The culture is then sterilized in the autoclave on three successive 

 days and filtered through a Pasteur filter. 



When an animal affected with glanders is inoculated (in the 

 horse,- usually in the subcutaneous tissue of the neck) with a suf- 

 ficient amount of mallein, the size of tbe dose varying according 

 to whether the mallein is concentrated or not (0.5 to 5 cubic 



ir This substance is prepared in Manila at the Government Serum Labo- 

 ratory and can be obtained by applying to the Bureau of Government 

 Laboratories. 



