21 



In another field of the section several small foci of infiltration 

 may be seen, situated at some distance beneath the pleural surface 

 and each is about the size of a single air cell. Their contents is 

 composed of cells similar to those found in the larger focus, but 

 karyorrhexis is not so marked a feature. One of these appears to 

 have ruptured into an adjoining alveolus, and such a process may 

 indicate the histogenesis of the larger foci. All the foci appear to 

 be recent ones, and there are no signs of proliferation or encapsula- 

 tion. No giant cells are to be seen. 



Spleen: This organ presents nothing noteworthy, excepting in- 

 tense congestion. 



Liver: There are no inflammatory foci, but throughout the sec- 

 tion the hepatic cells are granular and most of the nuclei stain 

 poorly or not at all. 



Kidneys : The blood vessels and glomeruli are congested through- 

 out. The cells lining the tubules are granular and many of their 

 nuclei stain poorly. No inflammatory foci are seen. 



Demonstration of Bacterium mallei in sections. It is generally 

 stated that the micro-organisms are stained in the tissues only 

 with the greatest difficulty. They were demonstrated very plainly 

 in sections of the lung nodules and skin pustules by Dr. Paul G. 

 Woolley, Director of the Serum Institute, by the following method : 



Paraffin sections of tissue fixed in Zenker: 



Remove paraffin with xylol. 



Absolute alcohol. 



95 per cent alcohol. 



80 per cent alcohol. 



Water. 



Tincture of iodin till well browned. 



80 per cent alcohol. 



Water. 



Immerse in Unna's alkaline methylene blue until purple. 



Absolute alcohol a second. 



Xylol. Wash and then immerse for ten minutes. 



Balsam. 

 Unna's alkaline methylene blue: 



Methylene blue, 1 gram. 



Potassium carbonate, 1 gram. 



Water, 100 grams. 



Steam in Arnold sterilizer for one-half hour to one hour. An essential 

 to success is that the dehydration with absolute alcohol must be performed 

 n^ rapidly as possible, a second or so being quite sufficient. 



