24 



Litmus mill:. The changes produced have varied on different 

 occasions. Usually no visible change is produced during two or 

 three days. In some instances slow acid production has subsequent- 

 ly appeared, with coagulation on the sixth or seventh day. The 

 cultures isolated from the human cases only reduced the litmus 

 in the milk and produced no further change during eight days' 

 observation. Control tubes remained unchanged. (This variation 

 may be due,, possibly, to changes produced in the milk before steri- 

 lization.) 



1 bouillon.- There is no sign of growth in the upper layers 

 of the fluid, but a slight, stringy precipitate rises if the test tube 

 is shaken. 



-\-l gelatin (containing muscle sugar) at 18 io 22. There 

 is nothing characteristic about the growth. A slight, whitish 

 growth appears along the stab and upon the surface. No liquefac- 

 tion occurs in ten days. 



Potato (unneutralizedj with a reaction of about -\-2). A luxu- 

 riant, moist, shining, yellowish-white growth appears in twenty- 

 four hours. This is quite viscous and in a few days takes on a 

 yellowish-brown color. (The growth is well described as resem- 

 bling a streak of honey ; but I have not noted any decided pigment 

 production during nine or ten days' growth, such as is described 

 by some observers.) 



-\-l glucose, lactose, and saccharose bouillon in the fermentation 

 tube. Only one culture was tested (human glanders, Case I, skin 

 pustule) . In this case good growth occurred in the bulb and open 

 neck of the fermentation tube, while the closed arm in each instance 

 remained clear. No test for changes in reaction were made. 



Morphology and polymorphism. The morphology of the organ- 

 ism varies considerably upon media of different reaction and 

 composition, as illustrated in Plate VI. Very minute rods usually 

 appear on +1 agar. On +1 glycerin agar and on potato the form 

 is more like that which is seen in preparations from the animal 

 body. On +1 agar containing 3 per cent salt (Cross and Black- 

 welPs table salt, which probably contained traces of nitrates) irreg- 

 ular, curved, and sinuous rods, with rounded ends or irregular, 

 branching forms appeared in one culture. Most of these rods, 

 when taken from a forty-eight-hour culture, stained evenly, though 

 some showed irregular vacuolation of the protoplasm. Clubbed 

 forms were seen. These involution forms were from 3.7 /u-10 /u, 



