No. 4.] REPORT OF CATTLE BUREAU. 331 



1911, a most ideal condition for the handling of interstate 

 cattle arriving there has been developed. The United States 

 Bureau of Animal Industry, early in the summer, offered 

 some suggestions relative to the manner of making the tuber- 

 culin test. As their formula did not seem to entirely fit the 

 requirements of Massachusetts, a circular of instructions for 

 making the tuberculin test was issued by the Bureau, having 

 first received the endorsement of the officials at Washington. 

 Copies of this circular were sent to all the agents of the 

 Cattle Bureau, and tlie testing of cattle is now being done in 

 a imiform manner throughout the State. The circular is as 

 follows : — 



Commonwealth of Massachusetts Cattle Bureau of the State 

 Board op Agriculture. 



Directions for making the Tuberculin Test. 



Make a physical examination of each animal, and give to each one 

 some designation by which it Avill be known throughout the test. 



Take each animal's temperature at least twice at two-hour intervals 

 on the day of injection. 



Use only such tuberculin as is furnished or apj^roved by this 

 Bureau or the United States Bureau of Animal Industry. 



If Bureau of Animal Industry tuberculin is used the dose is 2 

 cubic centimeters for an adult animal weighing about 750 pounds. 

 Yearlings and two-year-olds, according to size, should receive from 

 1 to 1^/4 cubic centimeters, while bulls and very large animals may 

 receive 3 cubic centimeters. Double or even triple doses should be 

 given to cattle recently injected with tuberculin ; also in the case of 

 animals which, on physical examination, are suspected of being 

 tuberculous. 



Begin taking temperatures not later than ten hours after injection, 

 and take them once in every two hours until the eighteenth hour is 

 reached, at which time if there is no tendency for the temperatui'e 

 to rise the test may cease. 



"When other than normal conditions are noticed about an animal 

 record should be made of the same. 



When retests of suspicious animals are made use double the origi- 

 nal dose. Begin taking temperatures not later than six hours after 

 injection, taking at least five temperatures at intervals of two hours, 

 at the expiration of which period if there is no tendency for the 

 temperature to rise the test may cease. 



Cattle tested within the limits of the State by order of the Chief 

 of the Cattle Bureau, and those tested outside the State by Massa- 



