20 HANDBOOK FOB BIO-CHEMICAL LABORATORY. 



but soluble in ether, and which contains 10.2$ platinum. It 

 is soluble in chloroform, carbon disulphide, benzol, and fatty 

 oils. 



Oxyhaemoglobin. 



Preparation. Defibrinated blood (best from the dog or the 

 horse) is mixed with at least 10 times its volume of a salt solu- 

 tion (1 vol. saturated solution with 9 vols. water), and allowed 

 to stand in a cool place for a couple of days until all the blood- 

 corpuscles have settled to the bottom. Remove the superna- 

 tant liquid by means of a siphon, and transfer the blood-cor- 

 puscles to a flask. Add 2 vols. water, and then shake with an 

 equal volume of ether. After a time remove the ether by de- 

 cantation, and allow the ether retained by the blood solution 

 to evaporate in an open dish in the air. Filter quickly 

 through a folded filter, cool the solution to C., and add while 

 stirring J of its volume of alcohol which has been cooled to 

 C. and allow to stand for several days at 5 to 10 C. 

 The crystals which separate are filtered off, pressed, and re- 

 crystallized by first quickly dissolving them in not too much 

 water at 20 to 30 C., cooling to C., precipitating with cold 

 alcohol as above, and allowed to stand at 5 to 10 C. This 

 recrystallization is repeated several times. Lastly, the crystals 

 are washed with cooled water containing alcohol ( vol. alco- 

 hol), and dried in vacuum at 0. or a lower temperature. 

 (Hoppe Seyler.) 



Properties. Oxyhaemoglobin from various kinds of blood 

 has different crystalline forms. It is insoluble in ether, chlo- 

 roform, benzol, and carbon disulphide. Crystals of oxyhaemo- 

 globin are insoluble in absolute alcohol. Its solution in water 

 is not precipitated by many metallic salts, but is precipitated 

 by sugar of lead. On heating the watery solution it decom- 

 poses at 60-70 C., and splits into hasmatin and albumin. It 

 is also decomposed by acids, alkalies, and many metallic salts. 



